Nephron Exp Nephrol

Background/Aims: SM22 ! , transgelin, has been revealed to be specifically expressed in glomerular epithelial cells and interstitial cells, according to the nature of the renal injury. In this study, quantitative analyses of SM22 ! positivity were performed to investigate the pathological significance of its expression. Methods: Kidney samples of adriamycin nephropathy underwent immunohistochemistry with a newly established anti-SM22 ! monoclonal antibody. The SM22 ! positivity was quantified by an image analyzer. The correlation of the histological values with biochemical data was investigated statistically. Microstructural localization of SM22 ! was studied by immunoelectron microscopy. Results: SM22 ! was expressed along the dense basal microfilaments of degenerating podocytes, and diffusely in interstitial cells. Both the extent and intensity of SM22 ! expression in glomerular and tubulointerstitial area were correlated with the deterioration of renal function and the severity of proteinuria. Stepwise multiple linear regression analysis revealed that the extent of its positivity in glomerular or tubuReceived: November 23, 2010 Accepted: April 29, 2011 Published online: December 23, 2011 Minoru Sakatsume, MD, PhD Division of Clinical Nephrology and Rheumatology Niigata University Graduate School of Medical and Dental Sciences 1-757 Asahimachi-dori, Niigata 951-8510 (Japan) Tel. +81 25 227 2193, E-Mail sakatsum @ med.niigata-u.ac.jp © 2011 S. Karger AG, Basel 1660–2129/12/1201–0001$38.00/0 Accessible online at: www.karger.com/nee © Free Author Copy – for personal use only ANY DISTRIBUTION OF THIS ARTICLE WITHOUT WRITTEN CONSENT FROM S. KARGER AG, BASEL IS A VIOLATION OF THE COPYRIGHT. Written permission to distribute the PDF will be granted against payment of a permission fee, which is based on the number of accesses required. Please contact [email protected] © Free Author Copy – for per sonal use only ANY DISTRIBUTION OF THIS ARTICLE WITHOUT WRITTEN CONSENT FROM S. KARGER AG, BASEL IS A VIOLATION OF THE COPYRIGHT. Written permission to distribute the PDF will be granted against payment of a per mission fee, which is based on the number of accesses required. Please contact [email protected]